Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species
نویسندگان
چکیده
OBJECTIVE Oxidative stress plays a key role in the pathophysiology of brain ischemia and neurodegenerative disorders. Previous studies indicated that Viola tricolor and Viola odorata are rich sources of antioxidants. This study aimed to determine whether these plants protect neurons against serum/glucose deprivation (SGD)-induced cell death in an in vitro model of ischemia and neurodegeneration. METHODS AND MATERIAL The PC12 neuronal cells were pretreated for 4 hr with 1 to 50 µg/ml of V. odorata or V. tricolor hydroalcoholic extracts followed by 24 hr incubation under SGD condition. Cell viability was evaluated by 4,5-dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide assay. The level of intracellular reactive oxygen species (ROS) was quantitated by flow cytometry using 2',7'- dichlorofluorescin diacetate as a probe. RESULTS SGD condition led to significant decrease in cell viability (p<0.001). Pretreatment with both V. tricolor and V. odorata extracts reduced the SGD-induced cytotoxicity. SGD resulted in a significant increase in intracellular ROS production (p<0.001). Both extracts at concentrations of 25 and 50 µg/ml could reverse the increased ROS production (p<0.05). CONCLUSION Results of the present study showed that V. tricolor and V. odorata protect neuronal cells against SGD-induced cell death, at least in part, by their antioxidant activities. Further studies on the possible application of these plants in prevention or treatment of cerebral ischemia and neurodegenerative diseases seem to be warranted.
منابع مشابه
Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species
Objective: Oxidative stress plays a key role in the pathophysiology of brain ischemia and neurodegenerative disorders.Previous studies indicated that Viola tricolor and Viola odorataare rich sources of antioxidants. This study aimed to determine whether these plants protect neurons against serum/glucose deprivation (SGD)-induced cell death in an in vitro model of ischemia and neurodegeneration....
متن کاملThe mechanism of neuroprotective effect of Viola odorata against serum/glucose deprivation-induced PC12 cell death
Objective: Oxidative stress is associated with the pathogenesis of brain ischemia and other neurodegenerative disorders. Previous researches have shown the antioxidant activity of Viola odorata L. In this project, we studied neuro-protective and reactive oxygen species (ROS) scavenging activities of methanol (MeOH) extract and other fractions isolated from <e...
متن کاملAmeliorative Effect of Viola odorata. L. Ethyl Acetate Extract against Nephrotoxicity Induced by Chronic Ethanol Exposure in Rats
Background and purpose: This study was designed to determine the protective effect of flower and leaf ethyl acetate extract of Viola odorata L. against ethanol induced chronic nephrotoxicity in male Wistar rats. Materials and methods: Ethyl acetate extracts of flower and leaf of V. odorata were prepared. Then, Total phenolic and flavonoid contents of extracts were determined. Animals were rand...
متن کاملAnti-melanogenic activity of Viola odorata different extracts on B16F10 murine melanoma cells
Objective(s): In previous studies, antioxidant activity of Viola odorata L. has been demonstrated. In this study, we have investigated the anti-melanogenic effect of extract and fractions of the plant in B16F10 cell line. Materials and Methods: Impact of different increasing concentrations of extract and fractions of V. odorata was evaluated on cell viability, cellular tyrosinase, melanin conte...
متن کاملاثر عصاره Viola odorata در کاهش نقصهای نورولوژیک و حجم سکته در مدل حیوانی ایسکمی کانونی مغزی
Background and purpose: Cerebral ischemia is a general injury characterized by direct tissue damage and increasing free radicals that leads to the death of brain tissue, cerebral infarction, or ischemic stroke. Some studies have demonstrated anti-inflammatory, antipyretic and antibacterial activities of Viola odorata. However, key aspects of the effect of Viola odorata in stroke are scarce. Thi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2016